Download a PDF version of the talk programme and poster programme.
08:30 | Registration opens |
09:00 | Welcoming opening address Ward De Spiegelaere, Ghent University, Belgium |
09:15 | Keynote lecture
Digital PCR, a technology for the future? - Jim Huggett, LGC and University of Surrey, UK |
9:50 |
Session 1: Technology
Towards a reproducible digital PCR data ecosystem with DDES: a community-supported digital PCR data essentials standard - Matthijs Vynck, Ghent University, Belgium The digital PCR Cross-Comparison study (dPCR-CroCo): a quantification benchmark - Wim Trypsteen, Ghent University, Belgium |
10:10 | Sponsored talk
Unleashing the potential of multiplexing in digital PCR applications on the QuantStudio Absolute Q digital PCR system - Tibor Füle, ThermoFisher Scientific (sponsored talk, ThermoFisher Scientific) Private mutation monitoring using the Digital LightCycler - Gertjan Wils, pxlence (sponsored talk, Roche) |
10:30 | Coffee break and poster viewing |
11:00 |
Session 2: Oncology
Invited talk:Optimizing and applying DNA methylation dPCR for liquid biopsy based monitoring of cancer - Heidi Pharo, Oslo University Hospital, Norway Invited talk: The use of dPCR to monitor response to treatment in cancer patients - Marzia Del Re, University of Pisa, Italy Surveillance of disease progression in metastatic breast cancer by molecular counting of circulating tumor DNA using Plasma SeqSensei-Breast IVD assay - Geert Martens, General Hospital Delta, Belgium Simultaneous detection of eight cancer types using a multiplex droplet digital PCR assay - Isabelle Neefs, Antwerp University Hospital, Belgium ESR1 mutation detection in FFPE and plasma DNA of breast cancer patients using Crystal Digital PCR™ and Nio+ - Cécile Jovelet, Stilla Technologies, France An in-house developed, sodium bisulfite-free, digital PCR-based method for quantification of DNA methylation biomarkers is valuable for diagnostic challenging early Sézary Syndrome patients - Willem Zoutman, Leiden University Medical Centre, Netherlands |
12:40 | Sponsored talk
Meet the Nio™+ - Alice Spenlehauer, Stilla Technologies (sponsored talk by Stilla Technologies) |
12:50 | Lunch and poster viewing |
13:50 |
Session 3: Methods
Invited talk: Digital melt analysis for broad-based, probe-free pathogen profiling - Stephanie Fraley, University of California San Diego, CA, USA Reporter emission multiplexing increases detection capacities of digital PCR devices leading to sensitive, precise and specific liquid biopsy assays - Silvia Calabrese, Hahn-Schickard-Gesellschaft für angewandte Forschung, Germany Allele-specific digital PCR enhances precision and sensitivity in the detection and quantification of copy number alterations in heterogeneous DNA samples - Rogier Nell, Leiden University Medical Center, Netherlands Technical comparison of quantitative, digital and real-time digital PCR instruments - David Gleerup, Ghent University, Belgium Total and encapsidated residual DNA in AAV production - Antonio Salgado, Exothera SA, Belgium Benchmarking digital PCR partition classification methods with empirical and simulated duplex data - Yao Chen, Ghent University, Belgium |
15:10 | Sponsored talk
Thinking outside the box: advanced applications of droplet digital PCR - Caroline Weydert, Bio-Rad (sponsored talk by Bio-Rad) Detecting FLT3-TKD mutations with double drop dPCR - Karl Vandepoele, Ghent University Hospital (sponsored talk by Qiagen) |
15:30 | Coffee break and poster viewing |
16:00 |
Session 4: Infectious diseases
Invited talk: The evolution of dPCR in the quantifications of viral reservoirs - Jori Symons, Univeristy Medical Center Utrecht, Netherlands Validation according to the ISO 20395:2019 of a digital PCR aimed at quantifying Salmonella enterica var. Infantis from poultry faeces - Matteo Richi, Istituto Zooprofilattico Sperimentale Lombardia e Emilia Romagna, Italy The Rainbow 5-plex digital PCR assay improves quantification of the viral reservoir - Mareva Delporte, Ghent University, Belgium |
16:50 | Sponsored talk
Advanced NIPT Detection of Trisomy Disorders Through Multiplexing Digital PCR Assay - Frank Lin, Sniper(sponsored talk by Sniper Medical Technology |
17:00 |
Session 5: Environmental, food and plant science
Invited talk: From field to fork - innovative applications of dPCR in plant breeding and food control - Antoon Lievens, BASF Innovation Centre, Belgium Implementation of multi-marker ddPCR analyses significantly increases reliability and precision of species detections and quantification in eDNA research - Rein Brys, Research Institute for Nature and Forest (INBO), Belgium Using dPCR and eDNA to delineate the spawning period of three commercially important fish species - Isolde Cornelis, Flanders Institute for Agriculture, Fisheries and Food, Belgium Advancing plant pathogen diagnostics: evaluation of a dPCR method for Xylella fastidiosa as a potential reference measurement procedure - Alexandra Bogožalec Košir, National Institute of Biology, Slovenia |
18:00 | Closing remarks Ward De Spiegelaere, Ghent University, Belgium Poster viewing with drinks and snacks |
19:00 | Close |
The specialist course on digital PCR will teach you the ins and outs of digital PCR, including assay and experiment design, assay optimization, data analysis, good experimental practice and data reporting. The course will offer hands-on sessions supported throughout by state of the art applications.
The course is taught by experts in the field of digital PCR from the Ghent University Digital PCR Center and some keynote speakers. Hands-on training on digital PCR devices is provided by Sniper (Sniper DQ24), Qiagen (QIAcuity), ThermoFisher (Absolute Q), Bio-Rad (QX600), Roche (digital LightCycler) and Stilla Technologies (Naica).
The course is open to both academic and non-academic researchers. Note that seats are limited to assure the course's quality and lecturer accessibility. See registration for pricing.
08:30-12:30 | Digital PCR principles and its (r)evolution since the late 1980s (prof. Ward De Spiegelaere, Ghent University) Title TBA (dr. Jim Huggett, LGC & University of Surrey) Designing internal controls for improved quantification with digital PCR (prof. Stephanie Fraley, University of California San Diego) Interpreting digital PCR data: from simple singleplex to highly multiplexed experiment (prof. Ward De Spiegelaere and dr. Matthijs Vynck, Ghent University) Instrument hands-on sessions (Bio-Rad, Qiagen, Roche, Sniper, Stilla, ThermoFisher) |
12:30-13:30 | Lunch and optional additional instrument hands-on (Bio-Rad, Qiagen, Roche, Sniper, Stilla, ThermoFisher) |
13:30-17:00 | Data interpretation and partition classification in digital PCR experiments: basics, automation, pitfalls; with hands-on training (dr. Wim Trypsteen, drs. Yao Chen, drs. David Gleerup, Ghent University) Instrument capabilities, software and applications (Bio-Rad, Qiagen, Roche, Sniper, Stilla, ThermoFisher) Digital PCR for DNA integrity and RNA analysis, and some exotic digital PCR applications (prof. Ward De Spiegelaere, Ghent University) |
08:30-12:30 | Digital PCR assay design from the ground up; theory and hands-on training (prof. Jo Vandesompele, Ghent University, CellCarta, pxlence) Optimizing and validating your digital PCR assays (dr. Antoon Lievens, BASF Innovation Centre) |
12:30-13:00 | Lunch |
13:00-16:00 | Automated partition classification in digital PCR experiments: a demonstration of the PoDCall algorithm (dr. Heidi Pharo, Oslo University Hospital) From sample to report: minimum information (dMIQE2020) and ISO guidelines for digital PCR experiments (prof. Ward De Spiegelaere, Ghent University) Statistical topics in digital PCR instrument and assay quality assessment; with hands-on training (dr. Matthijs Vynck, Ghent University) Beyond the basics: data analysis strategies for designing and analyzing digital PCR experiments (prof. Olivier Thas, Hasselt University, University of Wollongong, Ghent University) Advanced data analysis: hands-on training (dr. Matthijs Vynck, Ghent University, drs. Yao Chen, Ghent University) |
Abstract submission deadline oral presentations: Dec 15th 2023 23:59 CET Closed
Abstract submission deadline poster presentations: Jan 15th 2024 23:59 CET
We have finalized the program for invited, contributed and sponsored talks, but welcome subsmissions for contributed poster presentations. Please use the abstract template to prepare your abstract and submit it to the e-mail address provided within the template.
Interested in sponsoring the event? We offer a variety of sponsor packages. Please reach out to Wim Trypsteen.
Registration is closed
Akkerhage 10, 9000 Gent, Belgium
The venue is easily reachable by public transport in about 20 minutes. From Ghent's main train station (Gent-Sint-Pieters), walk towards the bus station just next to the train station (follow "bus" signs in the train station). Take bus 19 towards Arteveldepark from platform 10, exit at stop Gent Proeftuinstraat. Continue for a 3-minute walk in the travelling direction of the bus, taking the next turn right ("Akkerhage"). Continue for another 100m, you will see the venue on your lefthand side.
The venue is at approximately 15 minutes cycling from Ghent's main train station (Gent-Sint-Pieters) (directions). Blue bikes can be rented (membership required) at the rear entrance of the train station. You can also rent a bike from "de Fietsambassade" (no membership required), also located within walking distance from the rear entrance of the train station.
The venue is approximately 20 minutes by taxi from Ghent's main train station (Gent-Sint-Pieters). Taxis can be found at the front of the main train station, take a right upon exiting the train station's main entrance (prices and location on Visit Gent's website). For those travelling by car, the venue is easily reached from the main highways E17 and E40. The venue has on-site parking available (at the attendant's own expense).
The venue is an approximately 40 minute walk from Ghent's main train station (Gent-Sint-Pieters) (directions).
Looking for a place to stay?
The symposium and course is organized at the Van der Valk Hotel Gent (****). The venue offers a discount for the attendees, to get the discount code, e-mail ward.despiegelaere@ugent.be after registration.