Polytect: an automatic clustering and labeling method for multicolor digital PCR data

Polytect Builds on existing clustering methods, refining results by merging and labeling clusters based on prior knowledge of cluster center locations.
Polytect outperforms other methods in both empirical and simulated data, effectively merging excess clusters and identifying empty clusters.
The method is available as an R package and a Shiny app for easy use.

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Featured Publications

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Digital PCR threshold robustness analysis and optimization using dipcensR

DipcensR provides automates the assessment of partition classification robustness, reducing the need for extensive manual review.

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Review - Digital PCR Partition Classification

Summary of all available digital PCR partition classification approaches and the challenges

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The Digital MIQE Guidelines Update

Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

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Partition classification: ddpcRquant

Threshold determination for single channel droplet digital PCR experiments.

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Flexible data-analysis using GLMM

Flexible analysis of digital PCR experiments using generalised linear mixed models.

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Read all our dPCR related publications on PubMed

Key Publications from DIGPCR

2023, Clinical Chemistry
Review - Digital PCR Partition Classification
2022, Methods
Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA
2022, Human Genomics
Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA
2021, Clinical Chemistry
Digital Polymerase Chain Reaction for Assessment of Mutant Mitochondrial Carry-over after Nuclear Transfer for In Vitro Fertilization
2021, Analytical Biochemistry
Development of a 3':5' digital PCR assay to determine horse mRNA integrity
2020, Clinical Chemistry
The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
2018, Analytical and Bioanalytical Chemistry
On determining the power of digital PCR experiments
2018, Analytical Chemistry
Reducing Bias in Digital PCR Quantification Experiments: The Importance of Appropriately Modeling Volume Variability
2018, PLoS One
Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR
2018, Retrovirology
Review - Digital PCR as a tool to measure HIV persistence
2018, Clinical Genitourinary Cancer
Prognostic and Therapeutic Implications of Circulating Androgen Receptor Gene Copy Number in Prostate Cancer Patients Using Droplet Digital Polymerase Chain Reaction
2017, Analytical Chemistry
Model-Based Classification for Digital PCR: Your Umbrella for Rain
2017, Analytical and Bioanalytical Chemistry
Quality control of digital PCR assays and platforms
2016, Molecular Diagnosis and Therapy
Review - The future of digital polymerase chain reaction in virology
2016, Journal of Virus Eradication
Review - Diagnostic utility of droplet digital PCR for HIV reservoir quantification
2016, Biomolecular Detection and Quantification
Flexible analysis of digital PCR experiments using generalized linear mixed models
2015, Analytical and Bioanalytical Chemistry
ddpcRquant: threshold determination for single channel droplet digital PCR experiments
2015, Scientific Reports
Comparison of digital PCR platforms and semi-nested qPCR as a tool to determine the size of the HIV reservoir
2014, PLoS One
Comparison of Droplet Digital PCR and Seminested Real-Time PCR for Quantification of Cell-Associated HIV-1 RNA
2014, BMC Bioinformatics
Impact of variance components on reliability of absolute quantification using digital PCR
2013, Clinical Chemistry
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
2013, Analytical Biochemistry
Touchdown digital polymerase chain reaction for quantification of highly conserved sequences in the HIV-1 genome