Digital PCR threshold robustness analysis and optimization using dipcensR

Precise quantification of digital PCR data relies on accurate partition classification - Dividing dPCR partitions into "positive" and "negative" is critical for. However, partition classification can become very time-consuming as dPCR experiments scale up in throughput and complexity. Moreover, there are now good metrics to assess the reliability of the classification method.

DipcensR provides automates the assessment of partition classification robustness, reducing the need for extensive manual review. This helps make the dPCR data analysis workflow more efficient.

The tool includes an optional feature that can attempt to correct any aberrant partition classifications, further streamlining the analysis process.

DipcensR is available as an easy-to-use freely available R package.

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Featured Publications

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Review - Digital PCR Partition Classification

ummary of all available digital PCR partition classification approaches and the challenges

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The Digital MIQE Guidelines Update

Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

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Partition classification: ddpcRquant

Threshold determination for single channel droplet digital PCR experiments.

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Flexible data-analysis using GLMM

Flexible analysis of digital PCR experiments using generalised linear mixed models.

Key Publications from DIGPCR

2018, Analytical and Bioanalytical Chemistry
On determining the power of digital PCR experiments
2017, Analytical and Bioanalytical Chemistry
Quality control of digital PCR assays and platforms