Our Software solutions
Here you can find our software solutions, most are available as freely available web applications
Partition classification
ddpcRquant
Shiny web application and R code for single channel threshold setting. This tool will allow you to set a data driven treshold between positive and negative partitions in dPCR experiments
Polytect
Shiny web application and R code for single channel threshold setting. This tool will allow you to set a data driven treshold between positive and negative partitions in dPCR experiments
HIV DNA Rainbow Qiacuity
Shiny web application for analyzing HIV DNA Rainbow data from Qiacuity dPCR
Umbrella
R code for partition classification using the Umbrella method
DipcensR
Threshold robustness analysis and optimization
Statistical analysis tools
Flexible analysis for various calculations incorportating Standard error calculation in dPCR experiments
Shiny web application for standard error calculation using GLMM and Bootstrap methods. The applications includes absolute quantification, Copy number variation (for single and multiplex experiments), fractional abundance quantification and DNA shearing/Linkage analysis
Mixed models
SApplication for GLMM analysis of digital PCR experiments. This tool will help you to properly estimate confidence intervals in single and multiplex dPCR experiments
dPCalibRate
Analyzing the dynamic range and linearity of dilution curves. This tool can also be applied for qPCR experiments
DNA integrity calculation
Shiny web application for DNA integrity/linkage analysis by digital PCR
Other tools
DDES (Digital PCR Data Essentials Standard)
Shiny web application for converting and editing the DDES format
dPowerCalcR
Application for power calculation of absolute and relative digital PCR quantification experiments allowing the user to estimate the amounts of replicates needed based on expected concentrations, variation and partition number
Variance Components in dPCR
Mini website to simulate variance components in dPCR experiments, including effects of partition size variation, pipetting error and rain