Our Software solutions

Here you can find our software solutions, most are available as freely available web applications

Partition classification

ddpcRquant

Shiny web application and R code for single channel threshold setting. This tool will allow you to set a data driven treshold between positive and negative partitions in dPCR experiments

Polytect

Shiny web application and R code for single channel threshold setting. This tool will allow you to set a data driven treshold between positive and negative partitions in dPCR experiments

HIV DNA Rainbow Qiacuity

Shiny web application for analyzing HIV DNA Rainbow data from Qiacuity dPCR

Umbrella

R code for partition classification using the Umbrella method

DipcensR

Threshold robustness analysis and optimization

Statistical analysis tools

Flexible analysis for various calculations incorportating Standard error calculation in dPCR experiments

Shiny web application for standard error calculation using GLMM and Bootstrap methods. The applications includes absolute quantification, Copy number variation (for single and multiplex experiments), fractional abundance quantification and DNA shearing/Linkage analysis

Mixed models

SApplication for GLMM analysis of digital PCR experiments. This tool will help you to properly estimate confidence intervals in single and multiplex dPCR experiments

dPCalibRate

Analyzing the dynamic range and linearity of dilution curves. This tool can also be applied for qPCR experiments

DNA integrity calculation

Shiny web application for DNA integrity/linkage analysis by digital PCR

Other tools

DDES (Digital PCR Data Essentials Standard)

Shiny web application for converting and editing the DDES format

dPowerCalcR

Application for power calculation of absolute and relative digital PCR quantification experiments allowing the user to estimate the amounts of replicates needed based on expected concentrations, variation and partition number

Variance Components in dPCR

Mini website to simulate variance components in dPCR experiments, including effects of partition size variation, pipetting error and rain